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ecor i buffer  (Promega)

 
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    Promega ecor i buffer
    Ecor I Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecor+i+buffer/ecor+buffer/us07270969-1010-21-24
    Average 90 stars, based on 1 article reviews
    ecor i buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Methods of constructing and screening diverse expression libraries
    Article Snippet: Digestion of pDEATH-Trp vector used the following reaction conditions: pDEATH-Trp (25 μg) EcoR I Buffer (Promega) 100 μl BSA (10X) 100 μl EcoR I enzyme (20 U/μL) 4 μl H2O to 1000 μl Restriction digests were allowed to proceed for 5 minutes at 37° C. Samples were then purified using 3 QIAquick PCR purification columns as per manufacturer's instructions.

    Polymerase Chain Reaction:

    Article Title: Methods of constructing and screening diverse expression libraries
    Article Snippet: .. Restriction digests were completed in the following reactions: Digestion of PCR products used the following reaction conditions: PCR products (1 μg) EcoR I Buffer (Promega) 17 μl BSA (10x) 17 μl EcoR I enzyme (20 U/μL) (Promega) 0.9 μl H2O to 170 μl Restriction digests were allowed to proceed for 40 minutes at 37° C. Samples were then purified using QIAquick PCR purification columns as per manufacturer's instructions. ..

    Purification:

    Article Title: Methods of constructing and screening diverse expression libraries
    Article Snippet: .. Restriction digests were completed in the following reactions: Digestion of PCR products used the following reaction conditions: PCR products (1 μg) EcoR I Buffer (Promega) 17 μl BSA (10x) 17 μl EcoR I enzyme (20 U/μL) (Promega) 0.9 μl H2O to 170 μl Restriction digests were allowed to proceed for 40 minutes at 37° C. Samples were then purified using QIAquick PCR purification columns as per manufacturer's instructions. ..



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    Image Search Results


    Master mix for the enzymatic digestion with EcoR I

    Journal: Bio-protocol

    Article Title: Methylation-sensitive Amplified Polymorphism as a Tool to Analyze Wild Potato Hybrids

    doi: 10.21769/BioProtoc.3671

    Figure Lengend Snippet: Master mix for the enzymatic digestion with EcoR I

    Article Snippet: For the two reactions, calculate the required amount of each reagent for the total number of samples plus one, based on . table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Master mix H Reagents μl per sample EcoR I digestion 10 Buffer 1 10x 2 Hpa II 10 U/μl (NEB) 1 BSA 100x 0.2 H 2 O (ultrapure) 6.8 Final volume 20 Master mix M Reagents μl per sample EcoR I digestion 10 Buffer 4 10x 2 Msp I 20 U/μl (NEB) 0.5 BSA 100x 0.2 H 2 O (ultrapure) 7.3 Final volume 20 Open in a separate window caption a8 Master mixes for enzymatic digestions with Hpa II y Msp I Prepare the Master mixes, homogenize by vortexing (2-3 s) and centrifuge for 10 s. Distribute the Master mix H in the tubes labeled with the letter "H" and the Master mix M in the tubes labeled with the letter "M".

    Techniques:

    Master mixes for enzymatic digestions with Hpa II y Msp I

    Journal: Bio-protocol

    Article Title: Methylation-sensitive Amplified Polymorphism as a Tool to Analyze Wild Potato Hybrids

    doi: 10.21769/BioProtoc.3671

    Figure Lengend Snippet: Master mixes for enzymatic digestions with Hpa II y Msp I

    Article Snippet: For the two reactions, calculate the required amount of each reagent for the total number of samples plus one, based on . table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Master mix H Reagents μl per sample EcoR I digestion 10 Buffer 1 10x 2 Hpa II 10 U/μl (NEB) 1 BSA 100x 0.2 H 2 O (ultrapure) 6.8 Final volume 20 Master mix M Reagents μl per sample EcoR I digestion 10 Buffer 4 10x 2 Msp I 20 U/μl (NEB) 0.5 BSA 100x 0.2 H 2 O (ultrapure) 7.3 Final volume 20 Open in a separate window caption a8 Master mixes for enzymatic digestions with Hpa II y Msp I Prepare the Master mixes, homogenize by vortexing (2-3 s) and centrifuge for 10 s. Distribute the Master mix H in the tubes labeled with the letter "H" and the Master mix M in the tubes labeled with the letter "M".

    Techniques:

    Master mix for the ligation reaction

    Journal: Bio-protocol

    Article Title: Methylation-sensitive Amplified Polymorphism as a Tool to Analyze Wild Potato Hybrids

    doi: 10.21769/BioProtoc.3671

    Figure Lengend Snippet: Master mix for the ligation reaction

    Article Snippet: For the two reactions, calculate the required amount of each reagent for the total number of samples plus one, based on . table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Master mix H Reagents μl per sample EcoR I digestion 10 Buffer 1 10x 2 Hpa II 10 U/μl (NEB) 1 BSA 100x 0.2 H 2 O (ultrapure) 6.8 Final volume 20 Master mix M Reagents μl per sample EcoR I digestion 10 Buffer 4 10x 2 Msp I 20 U/μl (NEB) 0.5 BSA 100x 0.2 H 2 O (ultrapure) 7.3 Final volume 20 Open in a separate window caption a8 Master mixes for enzymatic digestions with Hpa II y Msp I Prepare the Master mixes, homogenize by vortexing (2-3 s) and centrifuge for 10 s. Distribute the Master mix H in the tubes labeled with the letter "H" and the Master mix M in the tubes labeled with the letter "M".

    Techniques: Ligation

    Master mix for the pre-amplification

    Journal: Bio-protocol

    Article Title: Methylation-sensitive Amplified Polymorphism as a Tool to Analyze Wild Potato Hybrids

    doi: 10.21769/BioProtoc.3671

    Figure Lengend Snippet: Master mix for the pre-amplification

    Article Snippet: For the two reactions, calculate the required amount of each reagent for the total number of samples plus one, based on . table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Master mix H Reagents μl per sample EcoR I digestion 10 Buffer 1 10x 2 Hpa II 10 U/μl (NEB) 1 BSA 100x 0.2 H 2 O (ultrapure) 6.8 Final volume 20 Master mix M Reagents μl per sample EcoR I digestion 10 Buffer 4 10x 2 Msp I 20 U/μl (NEB) 0.5 BSA 100x 0.2 H 2 O (ultrapure) 7.3 Final volume 20 Open in a separate window caption a8 Master mixes for enzymatic digestions with Hpa II y Msp I Prepare the Master mixes, homogenize by vortexing (2-3 s) and centrifuge for 10 s. Distribute the Master mix H in the tubes labeled with the letter "H" and the Master mix M in the tubes labeled with the letter "M".

    Techniques:

    Master mix for the amplification reaction

    Journal: Bio-protocol

    Article Title: Methylation-sensitive Amplified Polymorphism as a Tool to Analyze Wild Potato Hybrids

    doi: 10.21769/BioProtoc.3671

    Figure Lengend Snippet: Master mix for the amplification reaction

    Article Snippet: For the two reactions, calculate the required amount of each reagent for the total number of samples plus one, based on . table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Master mix H Reagents μl per sample EcoR I digestion 10 Buffer 1 10x 2 Hpa II 10 U/μl (NEB) 1 BSA 100x 0.2 H 2 O (ultrapure) 6.8 Final volume 20 Master mix M Reagents μl per sample EcoR I digestion 10 Buffer 4 10x 2 Msp I 20 U/μl (NEB) 0.5 BSA 100x 0.2 H 2 O (ultrapure) 7.3 Final volume 20 Open in a separate window caption a8 Master mixes for enzymatic digestions with Hpa II y Msp I Prepare the Master mixes, homogenize by vortexing (2-3 s) and centrifuge for 10 s. Distribute the Master mix H in the tubes labeled with the letter "H" and the Master mix M in the tubes labeled with the letter "M".

    Techniques: Amplification

    Master mix for the enzymatic digestion with EcoR I

    Journal: Bio-protocol

    Article Title: Methylation-sensitive Amplified Polymorphism as a Tool to Analyze Wild Potato Hybrids

    doi: 10.21769/BioProtoc.3671

    Figure Lengend Snippet: Master mix for the enzymatic digestion with EcoR I

    Article Snippet: For the two reactions, calculate the required amount of each reagent for the total number of samples plus one, based on . table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Master mix H Reagents μl per sample EcoR I digestion 10 Buffer 1 10x 2 Hpa II 10 U/μl (NEB) 1 BSA 100x 0.2 H 2 O (ultrapure) 6.8 Final volume 20 Master mix M Reagents μl per sample EcoR I digestion 10 Buffer 4 10x 2 Msp I 20 U/μl (NEB) 0.5 BSA 100x 0.2 H 2 O (ultrapure) 7.3 Final volume 20 Open in a separate window caption a8 Master mixes for enzymatic digestions with Hpa II y Msp I Prepare the Master mixes, homogenize by vortexing (2-3 s) and centrifuge for 10 s. Distribute the Master mix H in the tubes labeled with the letter "H" and the Master mix M in the tubes labeled with the letter "M".

    Techniques:

    Master mixes for enzymatic digestions with Hpa II y Msp I

    Journal: Bio-protocol

    Article Title: Methylation-sensitive Amplified Polymorphism as a Tool to Analyze Wild Potato Hybrids

    doi: 10.21769/BioProtoc.3671

    Figure Lengend Snippet: Master mixes for enzymatic digestions with Hpa II y Msp I

    Article Snippet: For the two reactions, calculate the required amount of each reagent for the total number of samples plus one, based on . table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Master mix H Reagents μl per sample EcoR I digestion 10 Buffer 1 10x 2 Hpa II 10 U/μl (NEB) 1 BSA 100x 0.2 H 2 O (ultrapure) 6.8 Final volume 20 Master mix M Reagents μl per sample EcoR I digestion 10 Buffer 4 10x 2 Msp I 20 U/μl (NEB) 0.5 BSA 100x 0.2 H 2 O (ultrapure) 7.3 Final volume 20 Open in a separate window caption a8 Master mixes for enzymatic digestions with Hpa II y Msp I Prepare the Master mixes, homogenize by vortexing (2-3 s) and centrifuge for 10 s. Distribute the Master mix H in the tubes labeled with the letter "H" and the Master mix M in the tubes labeled with the letter "M".

    Techniques:

    Master mix for the ligation reaction

    Journal: Bio-protocol

    Article Title: Methylation-sensitive Amplified Polymorphism as a Tool to Analyze Wild Potato Hybrids

    doi: 10.21769/BioProtoc.3671

    Figure Lengend Snippet: Master mix for the ligation reaction

    Article Snippet: For the two reactions, calculate the required amount of each reagent for the total number of samples plus one, based on . table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Master mix H Reagents μl per sample EcoR I digestion 10 Buffer 1 10x 2 Hpa II 10 U/μl (NEB) 1 BSA 100x 0.2 H 2 O (ultrapure) 6.8 Final volume 20 Master mix M Reagents μl per sample EcoR I digestion 10 Buffer 4 10x 2 Msp I 20 U/μl (NEB) 0.5 BSA 100x 0.2 H 2 O (ultrapure) 7.3 Final volume 20 Open in a separate window caption a8 Master mixes for enzymatic digestions with Hpa II y Msp I Prepare the Master mixes, homogenize by vortexing (2-3 s) and centrifuge for 10 s. Distribute the Master mix H in the tubes labeled with the letter "H" and the Master mix M in the tubes labeled with the letter "M".

    Techniques: Ligation

    Master mix for the pre-amplification

    Journal: Bio-protocol

    Article Title: Methylation-sensitive Amplified Polymorphism as a Tool to Analyze Wild Potato Hybrids

    doi: 10.21769/BioProtoc.3671

    Figure Lengend Snippet: Master mix for the pre-amplification

    Article Snippet: For the two reactions, calculate the required amount of each reagent for the total number of samples plus one, based on . table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Master mix H Reagents μl per sample EcoR I digestion 10 Buffer 1 10x 2 Hpa II 10 U/μl (NEB) 1 BSA 100x 0.2 H 2 O (ultrapure) 6.8 Final volume 20 Master mix M Reagents μl per sample EcoR I digestion 10 Buffer 4 10x 2 Msp I 20 U/μl (NEB) 0.5 BSA 100x 0.2 H 2 O (ultrapure) 7.3 Final volume 20 Open in a separate window caption a8 Master mixes for enzymatic digestions with Hpa II y Msp I Prepare the Master mixes, homogenize by vortexing (2-3 s) and centrifuge for 10 s. Distribute the Master mix H in the tubes labeled with the letter "H" and the Master mix M in the tubes labeled with the letter "M".

    Techniques:

    Master mix for the amplification reaction

    Journal: Bio-protocol

    Article Title: Methylation-sensitive Amplified Polymorphism as a Tool to Analyze Wild Potato Hybrids

    doi: 10.21769/BioProtoc.3671

    Figure Lengend Snippet: Master mix for the amplification reaction

    Article Snippet: For the two reactions, calculate the required amount of each reagent for the total number of samples plus one, based on . table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Master mix H Reagents μl per sample EcoR I digestion 10 Buffer 1 10x 2 Hpa II 10 U/μl (NEB) 1 BSA 100x 0.2 H 2 O (ultrapure) 6.8 Final volume 20 Master mix M Reagents μl per sample EcoR I digestion 10 Buffer 4 10x 2 Msp I 20 U/μl (NEB) 0.5 BSA 100x 0.2 H 2 O (ultrapure) 7.3 Final volume 20 Open in a separate window caption a8 Master mixes for enzymatic digestions with Hpa II y Msp I Prepare the Master mixes, homogenize by vortexing (2-3 s) and centrifuge for 10 s. Distribute the Master mix H in the tubes labeled with the letter "H" and the Master mix M in the tubes labeled with the letter "M".

    Techniques: Amplification